Amylase CPT: 82150

Expected Turnaround Time

Within 1 day

Specimen Requirements


Serum (preferred) or plasma


1 mL

Minimum Volume

0.5 mL


Red-top tube, gel-barrier tube, or green-top (heparin) tube


Separate serum or plasma from cells within 45 minutes of collection.

Storage Instructions

Maintain specimen at room temperature.

Stability Requirements

Temperature Period
Room Temperature7 days
Refrigerated1 month
FrozenNot recommended
Freeze/thaw/ cycles Stable x 3

Patient Information

Primarily to diagnose and monitor acute pancreatitis; also sometimes to diagnose and monitor chronic pancreatitis or other pancreatic diseases.

When you have symptoms of a pancreatic disorder, such as severe abdominal pain, fever, loss of appetite, or nausea.

A blood sample is obtained by inserting a needle into a vein in the arm. Sometimes a random urine sample, a 24-hour urine sample, or peritoneal fluid is collected.

For both a blood test and a urine sample, you should avoid alcohol for 24 hours before the test and for the blood test, you may be instructed not eat or drink anything other than water for two hours before blood is drawn. Certain medications can affect amylase levels, so discuss medications with your doctor before testing is done.