Bilirubin, Direct CPT: 82248

Expected Turnaround Time

Within 1 day

Specimen Requirements


Serum (preferred) or plasma


1 mL

Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)


Red-top tube, gel-barrier tube, or green-top (lithium heparin) tube.


Separate serum or plasma from cells within 45 minutes of collection.

Storage Instructions


Stability Requirements

Temperature Period
Room Temperature 2 days
Refrigerated 3 days
Frozen 14 days
Freeze/thaw/ cycles Stable x 3

Patient Information

To screen for or monitor liver disorders or hemolytic anemia; to monitor neonatal jaundice.

When you have signs or symptoms of liver damage, liver disease, bile duct blockage, hemolytic anemia, or a liver-related metabolic problem, or if a newborn has jaundice.

In adults, a blood sample drawn from a vein in the arm; in newborns, a blood sample from a heelstick; non-invasive technology is available in some healthcare facilities that will measure bilirubin by using an instrument placed on the skin (transcutaneous bilirubin meter).

You may need to fast (nothing but water) for several hours before the test; fasting requirements vary by laboratory; ask your lab or healthcare provider for instructions.