High-density Lipoprotein Cholesterol (HDL-C) CPT: 83718


Alpha-Lipoprotein Cholesterol

HDL Cholesterol

HDL Cholesterol Electrophoresis


Expected Turnaround Time

Within 1 day

Specimen Requirements


Serum (preferred) or plasma.


1 mL

Minimum Volume

0.5 mL


Separate serum or plasma from cells within 45 minutes of collection.


Separate serum or plasma from cells within 45 minutes of collection.

Storage Instructions

Maintain specimen at room temperature.

Stability Requirements

Temperature Period
Room Temperature 3 days
Refrigerated 14 days
Frozen 14 days
Freeze/thaw/ cycles Stable x 2

Patient Information

Patient Preparation

Patient should be on a normal diet and maintain a stable weight for a week prior to testing. Any drugs should be discontinued for three to four weeks if possible. Test should not be performed until three months after a myocardial infarction or similar traumatic episode, such as severe infection or inflammation. Fasting is not necessary.

To determine your risk of developing heart disease.

Screening: as part of a regular health exam with a lipid profile when no risk factors for heart disease are present; once every four to six years in adults; children should have a lipid profile screening at least once between the ages of 9 and 11 and then again between the ages of 17 and 21.

Monitoring: may be done more frequently and at regular intervals as part of a lipid profile when risk factors for heart disease are present, when prior results showed high risk levels, and/or when undergoing treatment for unhealthy lipid levels.

A blood sample drawn from a vein in your arm or from a fingerstick.

If this test is to be performed as part of a complete lipid profile, fasting for 9 to 12 hours typically will be required; only water is permitted. Your healthcare practitioner may decide that you may be tested without fasting. Follow any instructions you are given and tell the person drawing your blood whether or not you have fasted. For youths without risk factors, testing may be done without fasting.