Progesterone CPT: 84144

Special Instructions

This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.

Expected Turnaround Time

Within 1 day

Specimen Requirements


Serum (preferred) or plasma (lithium heparin only). EDTA plasma causes decreased results.


0.8 mL


Red-top tube or gel-barrier tube.


If a red-top tube is used, transfer separated serum to a plastic transport tube.

Storage Instructions

Maintain specimen at room temperature.

Stability Requirements

Temperature Period
Room Temperature Not recommended
Refrigerated 7 days
Frozen 4 weeks
Freeze/thaw/ cycles Stable x 3

Patient Information

To help determine the cause of infertility, track ovulation, help diagnose an ectopic or failing pregnancy, monitor the health of a pregnancy, monitor progesterone replacement therapy, or help diagnose the cause of abnormal uterine bleeding.

At specific times during a woman’s menstrual cycle to determine whether/when she is ovulating (releasing an egg from an ovary); during early pregnancy when symptoms suggest an ectopic or failing pregnancy; throughout a high-risk pregnancy to help determine placenta and fetal health; periodically when a person is receiving progesterone replacement therapy; when a woman has abnormal uterine bleeding.

A blood sample drawn from a vein in your arm.

None, but for women, the date of your last menstrual period or trimester of pregnancy should be noted.